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The contaminated soil is prepared for treatment by creating fractures and fissures therein using fluid pressure introduced through spaced apart holes drilled to selected depths. The depth of the drilled holes and the hole spacing will depend on the area of contamination and soil type. The Isolite with microbes stored within the internal pores is then injected into the spaces of the fractures and fissures using air or liquid pressure. The internal pore space of the diatomaceous earth pellets may range in size from 0.1 to 1.0 microns or larger. When the inoculated pellets are in place, the microbes have a permanent home to receive and effectively eliminate the contaminates. The drilled holes may also be retrofitted with perforated pipe for supplementing the microbes with additional oxygen, water and nutrients or adding more pellets with microbes therein for completing the clean-up of the contaminated soil and ground water.
BioNet™ is a system for in-situ bioremediation of contaminated soil and ground water wherein Isolite (porous inorganic support spheres of diatomaceous earth pellets) are treated by inoculation with selected non-pathogenic microbes, i.e. bacteria, for converting or destroying the contaminates. The contaminates may be hydrocarbons, sulphides, frac flow back water, produced water, cyanides, nitrates, heavy metals and other chemicals. The porous cells of the Isolite provide a home not only for the microbes but also for oxygen, water and other nutrients to help sustain the life of the augmented colonies of microbes introduced in the Isolite.